Induction of Erythropoiesis by MHC-Mediated Cognate Interactions between B- and T-Cells

Authors

A Guha, Departments of Medicine and Pathology , University of Connecticut Health Center , Farmington , CT and Department of Medicine , Bridgeport Hospital , Bridgeport , CT.
D P. Tuck, Departments of Medicine and Pathology , University of Connecticut Health Center , Farmington , CT and Department of Medicine , Bridgeport Hospital , Bridgeport , CT.
R E. Cone, Departments of Medicine and Pathology , University of Connecticut Health Center , Farmington , CT and Department of Medicine , Bridgeport Hospital , Bridgeport , CT.
N Dainiak, Departments of Medicine and Pathology , University of Connecticut Health Center , Farmington , CT and Department of Medicine , Bridgeport Hospital , Bridgeport , CT.

Document Type

Article

Publication Title

Hematology (Amsterdam, Netherlands)

Abstract

We have previously shown that the expression of membrane burst-promoting activity (mBPA), an erythropoietic cytokine, by B-lymphocytes is augmented by the addition of allogeneic effector cells to the B-cells. Here, we have examined immune mechanisms involved in the induction/promotion of erythropoiesis as assessed by the capacity of autologous and allogeneic peripheral blood lymphocytes to augment burst-forming unit-erythroid (BFU-E) in normal human bone marrow cells in vitro. Preincubation of mBPA-expressing human B-cells with monoclonal antibodies to major histocompatibility complex (MHC) antigens, abrogated erythropoietic activity of both autologous and allogeneic lymphocytes, suggesting that MHC antigens play a role in regulating the expression of the erythroid growth factor. Inhibition of BFU-E proliferation was also evident when antibodies to MHC class-I or class-II antigens were added directly to marrow culture. Furthermore, addition of anti-CD4 antibody to the cultures of PBL and autologous target BM cells markedly reduced erythroid proliferation induced by PBL. By contrast, anti-CD8 and control (UPC-10) monoclonal antibodies had no effect. These results provide evidence that MHC-mediated cognate interactions between T- and B-lymphocytes may participate in the control of erythropoiesis, either directly or by modulating mBPA function.

First Page

29

Last Page

38

DOI

10.1080/10245332.1997.11746317

Publication Date

1-1-1997

Recommended Citation

Guha, A; Tuck, D P.; Cone, R E.; and Dainiak, N, "Induction of Erythropoiesis by MHC-Mediated Cognate Interactions between B- and T-Cells" (1997). All Research. 410.
https://scholar.bridgeporthospital.org/all_research/410

Identifier

27406724 (pubmed); 10.1080/10245332.1997.11746317 (doi)