Tenascin is highly expressed in endometriosis and its expression is upregulated by estrogen

Authors

Orkun Tan, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut; Department of Obstetrics and Gynecology, Bridgeport Hospital, Bridgeport, Connecticut.
Turkan Ornek, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut; Department of Obstetrics and Gynecology, Ufuk University School of Medicine, Ankara, Turkey.
Yasemin Seval, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut; Department of Histology and Embryology Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Leyla Sati, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut; Department of Histology and Embryology Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Aydin Arici, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut. Electronic address: aydin.arici@yale.edu.

Document Type

Article

Publication Title

Fertility and sterility

Abstract

OBJECTIVE: To investigate the localization of tenascin expression in the endometrium of women without endometriosis and in endometriotic implants, and to determine the in vitro regulation of tenascin by E(2) in these tissues. DESIGN: Experimental laboratory study. SETTING: University medical center. PATIENT(S): Reproductive age women with or without endometriosis. INTERVENTION(S): Proliferative (n = 14), and secretory (n = 14) endometrium from women without endometriosis and endometriosis implants (n = 14) were used for immunohistochemical analysis. Endometrial and endometriotic stromal cells were grown in culture and treated with E(2), the estrogen receptor antagonist ICI 182 780 (ICI) alone, E(2) in combination with ICI, or vehicle (control) for 24 hours, and tenascin expression was analyzed by Western blotting. MAIN OUTCOME MEASURE(S): Expression levels of tenascin in normal endometrium and endometriotic implants and its regulation by E(2). RESULT(S): Tenascin immunostaining revealed an increasing intensity in the stromal cells, starting from normal secretory endometrium, then normal proliferative endometrium, and reaching the highest expression in endometriotic implants. Estradiol induced a significant increase in tenascin protein levels in the endometriotic stromal cells in culture. CONCLUSION(S): The modulation of tenascin as an extracellular matrix protein by E(2) in endometriotic stromal cells may be one of the factors playing a role in the development of endometriosis.

First Page

1082

Last Page

1089

DOI

10.1016/j.fertnstert.2007.05.028

Publication Date

5-1-2008

Recommended Citation

Tan, Orkun; Ornek, Turkan; Seval, Yasemin; Sati, Leyla; and Arici, Aydin, "Tenascin is highly expressed in endometriosis and its expression is upregulated by estrogen" (2008). Internal Medicine. 192.
https://scholar.bridgeporthospital.org/internal_medicine/192

Identifier

17681310 (pubmed); 10.1016/j.fertnstert.2007.05.028 (doi); S0015-0282(07)01153-3 (pii)